The eukaryotic MEP-pathway genes are evolutionarily conserved and originated from Chlaymidia and cyanobacteria

Abstract Background Isoprenoids are the most ancient and essential class of metabolites produced in all organisms, either via mevalonate (MVA)-and/or methylerythritol phosphate (MEP)-pathways. The MEP-pathway is present plastid-bearing organisms eubacteria. However, no comprehensive study reveals origination evolutionary characteristics genes eukaryotes. Results Here, detailed bioinformatics analyses provide an in-depth understanding history this indispensable biochemical route, offer a basis for co-existence cytosolic MVA- plastidial plants given established exchange end products between two isoprenoid-biosynthesis pathways. phylogenetic establish contributions both cyanobacteria Chlamydiae sequences to plant’s genes. Moreover, Phylogenetic inter-species syntenic block demonstrate that six seven have predominantly remained as single-copy land spite multiple whole-genome duplication events (WGDs). Substitution rate domain studies display conservation these genes, reinforced by their high expression levels. Distinct phenotypic variation among with reduced levels individual confirm function each nuclear-encoded plastid-targeted enzyme plant growth development. Conclusion Collectively, findings reveal polyphyletic origin restrict reinforce potential enzymes beyond production isoprenoids intermediates.